SELECTIVELY IMPROVEMENT OF THE RESPONSE OF IMMUNE CELLS TO OPPORTUNISTIC MICROORGANISMS: AN INNOVATIVE PROPOSAL FOR IBD FROM AN ANCIENT GRAIN

Abstract BACKGROUND Inflammatory Bowel Disease (IBD) results from an unbalanced immune response to the gut microbiome. There is a lack of proposals that integrate and may balance multiple factors (Immunity, microbiome diet) together condition onset evolution disease. D-fagomine small molecule, naturally present in buckwheat grain. It Is monosaccharide mimetic showing glycosidase enzyme inhibition properties. Clinical studies have shown its efficacy control postprandial glycemic response. Subsequent research “in vitro” had also inhibit fimbriae adhesion biofilm formation some proteobacteria, including ie E. coli or Salmonella. ORIGINAL AIM The aim study was confirm effects on human mucosa using adherent-invasive E (AIEC) microorganism usually linked IBD. METHOD Ex vivo resected colonic cultured (4 h) with AIEC inoculum at different range doses (0-5000 ppm). amount tissue supernatant quantified, measuring TNF-alfa LDH. RESULTS confirmed direct dose-response relationship. Totally unexpected, found modulate apparent double sigmoid curve max activity 50 ppm (Figure 1). This corresponds activator innate cells mechanism related complement activation, (ie mannose, betaglucan). Finally helping detect overcome evasion ppm. Additional were considered necessary these models. ADDED novel assess cell (other than mucosa) dose front microorganisms able evade system. blood (50 ppm) correspondent models: bacteria = fungus Blastomyces sp. quantified. D-fagomine, ppm, stimulates against 2). Both models give coherent results, improves capacity opportunistic microorganisms. CONCLUSIONS Our for first time develop more efficient better presence great interest IBD synergic delaying release sugars diet gut, reducing then negative Western Diet humans